Plasmid

Part:BBa_K2418003:Design

Designed by: Akos Sukosd   Group: iGEM17_Szeged_SA_RMG   (2017-10-26)


The full sequence of the modular broad-host-range expression vector pMHE7


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 3280
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 175
    Illegal BamHI site found at 408
    Illegal XhoI site found at 312
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 143
    Illegal NgoMIV site found at 1008
    Illegal NgoMIV site found at 4403
    Illegal AgeI site found at 848
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5428
    Illegal BsaI.rc site found at 3602


Design Notes

The LDH gene (BBa_K2418000) and the moxY promoter (BBa_K2418001; BBa_K2418002) was inserted into the modular broad-host range vector pMHE5.


Source

Synthetized sequence

References